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PCR Polishing Kit

Agilent Technologies200409

PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.

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Description

Description

PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.

Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilizing blunt-ended ligation.

Features:

Generates blunt-ended DNA fragments in 30 minutes
More efficient than Klenow polishing
Optimizes blunt-end cloning efficiencies

For Research Use Only. Not for use in diagnostic procedures.

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PCR Polishing Kit - Agilent Technologies - Life Sciences

PCR Polishing Kit

Agilent Technologies

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