NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc).

The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

NEBNext® Ultra™ DNA Library Prep Kit for Illumina® Features:

• Input amounts as low as 5ng
• Fast workflow (<3 hours), with minimal hands-on time
• Robust, reliable performance
• Ultra high fidelity amplification with minimized GC bias

NEBNext® Ultra™ DNA Library Prep Kit for Illumina® Includes:

• NEBNext End Prep Enzyme Mix
• NEBNext End Repair Reaction Buffer (10X)
• Blunt/TA Ligase Master Mix
• NEBNext Ligation Enhancer
• NEBNext High-Fidelity 2X PCR Master Mix (2X)