microCLEAN
microCLEAN is part of the Microzone Free From range of products. There are no filter columns, magnetic beads, alcohols nor solvents used. microCLEAN removes unwanted residual dNTPs, short primer dimers, unincorporated dyes etc post PCR. Quick and simple protocol with minimal equipment required.
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Very reliable. Very clean PCR products resulting in excellent Sanger Sequencing.
Clean up of PCR reactions
Our molecular diagnostic laboratory has been using Microlean for cleaning up our PCR reactions prior to Sanger sequencing for many years. I would highly recommend it for ease of use and reliability. We have never had a 'loss of product' incident. And our sequencing reactions are reproducibly clean. We have advised other departments within our insitution to move to this product compared to other platforms.
Review Date: 13 Dec 2023 | Microzone
MicroCLEAN is part of the Microzone Free From range of products. It does not use filter columns, magnetic beads, alcohols, solvents.
MicroCLEAN used on Post PCR reactions will remove unwanted residual dNTPs, short primer dimers, unincorporated dyes etc.
Quick and simple protocol with minimal equipment required.
Key Features
- Simple method without the need for filter columns or magnetic beads.
- Less environmental impact from plastics.
- Free from the use of alcohols and other solvents.
- Easy method allows MicroCLEAN to be used outside of a laboratory and in the field.
Applications
- Post PCR Clean Up of dsDNA
Preparation of bacterial samples for sequencing
It is estimated that hospital-associated infections (HAI) cost between $35 and $45 billion in the USA and the NHS £3 billion in the UK, as well as countless lives. To counter such costs, Microzone proposes a method for the rapid preparation of cultured bacterial samples for Sanger Sequencing. Utilization of Microzone's 'free from' technology is highlighted for allowing for fast, efficient workflows only requiring a centrifugation step and not requiring automation.
