MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit
For quantitation of global DNA methylation via a one-step ELISA method
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The MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, "one-step" ELISA-like reaction. This allows global DNA methylation to be directly measured more quickly than other techniques such as LUMA, LINE-1, Alu or LTR-based assays.
As a fourth generation technology of EpiGentek's patented global DNA methylation technique, it is a further refinement of the popular, predecessor MethylFlash kit by improving upon speed, simplicity, sensitivity, and reproducibility.
This kit is also specifically optimized for paired use with the MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.
This kit has the following advantages and features:
- Fast - Reduced steps so that the entire procedure only needs 2 hours
- Robust - Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
- Convenient - Inherently low background noise, thereby eliminating the need for DNA denaturation and plate blocking steps
- Sensitive - Detection limit can be as low as 0.05% methylated DNA from 100 ng of input DNA
- Specific - High specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA
- Universal - Positive and negative controls are included and allow for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA
- Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis
- Flexible - Strip-well microplate format makes the assay available for manual or high throughput analysis
