Mannitol Salt Agar
Mannitol Salt Agar is used for the isolation of staphylococci. Conforms to Harmonized USP/EP/JP Requirements. Chapman formulated Mannitol Salt Agar to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration. Chapman added 7.5% Sodium Chloride to Phenol Red Mannitol Agar, and noted pathogenic strains of staphylococci (coagulase-positive staphylococci) grew luxuriantly and produced yello…
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Mannitol Salt Agar is used for the isolation of staphylococci. Conforms to Harmonized USP/EP/JP Requirements.
Chapman formulated Mannitol Salt Agar to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration. Chapman added 7.5% Sodium Chloride to Phenol Red Mannitol Agar, and noted pathogenic strains of staphylococci (coagulase-positive staphylococci) grew luxuriantly and produced yellow colonies with yellow zones. Nonpathogenic staphylococci produced small red colonies with no color change to the surrounding medium.
Mannitol Salt Agar is highly selective, and specimens from heavily contaminated sources may be streaked onto this medium without danger of overgrowth. Mannitol Salt Agar is recommended for isolating pathogenic staphylococci from clinical specimens, cosmetics, and microbial limit tests.
