MACSQuant® VYB Analyzer
Your benchmark for fluorescent protein flow cytometry
MACSQuant VYB - Flow Cytometer
MACSQuant VYB - Automated Analysis
MACSQuant VYB - Optical Bench
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Excellent machine - easy to use. Would definitely recommend.
flow machine
Excellent machine - easy to use. I would definitely recommend it. Miltenyi has validated Abs which makes it easier to design and use flow panels. The auto compensation feature is very useful as well. Also ready-made buffers.
Review Date: 8 Jul 2022 | Miltenyi Biotec B.V. & Co. KG
excellent machine - easy to use. would definitely recommend.
flow machine
excellent machine - easy to use. would definitely recommend. Miltenyi has validated Abs which makes it easier to design and use flow panels. auto compensation feature is very useful as well. also readymade buffers.
Review Date: 8 Jul 2022 | Miltenyi Biotec B.V. & Co. KG
We love our MaxQuant!
Flow cytometry
Great machine which has a small footprint and completely automated. Samples run reliably and it is a please to use.
Review Date: 11 Feb 2022 | Miltenyi Biotec B.V. & Co. KG
Small size, fixed laser instrument for basic analysis. Would not buy again though.
Flow Cytometry
Nice small size fixed laser analyzer, easy to use. Can also do cell/event count in a defined volume. File transfer a bit convoluted but doable! Have had to interact quite a bit with tech support, service and sales in the past 2 years with continuous problems with alignment of lasers slipping gradually over a 4-6 month span requiring service, so definitely keep your service contract up to date!
Review Date: 10 Nov 2020 | Miltenyi Biotec B.V. & Co. KG
Small bench top FACS that is robust and easy to use - highly recommended!
Flow Cytometry
For the MACsQuant or the Vyb as we call it, is a solid work horse of the lab. It goes from 9am to 4pm non-stop most days. Our use is mainly characterization of hematopoietic cells and the occassional adherent cell line. For 3 colour analysis its perfect. We have the three laser version and there is no real need for compensation if you use the vioBlue, FITC (GFP) and then the Y4 (PE/APC-Vio770 conjugated antibodies) channels. Miltenyi has a great selection of antibodies as well. We also do four colours very simply when we have mCherry which is detected in the Y2 channel. It can also do high throughput and we routinely run 96 well plates. Note - if you do not have the optional refilling station the running buffer will run out about 3/4 of the way through so you need to be there to replace it. The FACS also has a mix option as well so there is no problem with the cells settling in the bottom of the well either. Service: we have had no serious issues with the machine. Only once did we have serious blockage and at one stage one of the valves started to leak. But all were solved in a timely manner. They do come and update the software now and again but this also means you lose your data so you need to back this up. Analysis: we do all this in FlowJo. You do need to do Miltenyi Transform to see the cells but otherwise that's the only tweak required. Do note that the resolution of populations is not as good as a high end BD machine. But I guess this is why this machine is essentially 1/4 the price?! One nice feature is that it gives you a cell concentration as well because it takes a certain volume for each sample rather than continuously aspirate until it gets the number of stated events. All in all my impression from using it the last few years is that it is a great machine for all our routine 3-4 colour work.
Review Date: 3 Mar 2017 | Miltenyi Biotec B.V. & Co. KG
Fast, rapid, fully automated multi-color flow cytometry
Identity and characterization of all cells types dendritic cells, endothelial, T cells, tumor cells
There are three lasers (405, 488, 561 nm), two scatter (FSC, SSC) and eight fluorescent channels, this allows for good flow cytometry results as compared to other 2 laser systems. It is fully automatic and there are cool color codes for each reagent which light up like mini disco lights. It is fast, cell counts are available in less than a minute,
Review Date: 25 Jan 2017 | Miltenyi Biotec B.V. & Co. KG
Cell Analysis, protein expression, fluorescent proteins screening
We bought the instrument for screening samples for expression of fusion proteins with fluorescent proteins. Its clever laser design allows detection of 4 different fluorescent proteins at the same time, reducing time analysis and increasing throughput. FCS files can be read in any flow cytometry analysis software. But currently we are using it for a lot of different applications, from cell cycle analysis from fish or plants to blood immunophenotyping.
Review Date: 17 Jul 2015 | Miltenyi Biotec B.V. & Co. KG
Cell phenotyping, reporter gene expression analyses
The machine is fine. We had some issues with the one we bought, several components had to be changed, eventually it got much more stable, the service engineers also got way more experience. Original software did not allow for much flexibility, but a new version came out and presumably some issues got fixed. Steep learning curve, but once you get some experience the setup is quite straightforward. 96 well plate sampler is a handy feature. Because of our experiments specifics, we need a flow cytometer in a flow cabinet. Macsquant is compact enough to fit in one.
Review Date: 14 Jul 2015 | Miltenyi Biotec B.V. & Co. KG
Flow Cytometry
It is easy to use for automated sampling with a 96-well format. Automated staining of samples is possible. The after sale care is very good. However the software performance could be enhanced. An update to flowjo version 10 is a good step for the future improvements. It is very good value for money.
Review Date: 21 Nov 2014 | Miltenyi Biotec B.V. & Co. KG
My lab uses this machine and we have never had an issue with it. After-purchase services were never a problem.
Review Date: 3 Jun 2013 | Miltenyi Biotec B.V. & Co. KG
Flow cytometry with the MACSQuant Family of flow cytometers is all about performance, automation, compactness, and convenience. The MACSQuant VYB not only combines these features, but also comes with a uniquely configured optical bench, featuring violet, yellow, and blue lasers. It is a perfect match for detecting fluorescent protein reporters or better discrimination between PE and FITC conjugates.
Technology highlight
Finding the right fit for fluorescent proteins with the yellow laser
- Fully utilize fluorescent proteins and reporter applications that require the Violet, Blue, or Yellow laser such as mCherry, GFP and CFP
- Optimal excitation of PE and PE tandem dyes
- Excite and analyse APC and APC tandems
- Minimize compensation for FITC/GFP and PE conjugates
- 21 CFR Part 11 compliant software option
Product Specs:
- Quantity: EA
- Dimensions: (WxDxH* *adjustable touch screen) 669x400x394-553.5mm
- Applications: Flow Cytometry
- Excitation:
- 405nm (violet)
- 488nm (blue)
- 640nm (red)
- Detection: FSC, SSC, 8 fluorescence channels
- Regulatory status: For research use only
- Sample format: 1.5/2.0/5.0/15/50 mL tubes or 24/48/96-well plates
- Measurement speed: < 25 minutes per 96-well plate (5 µL measurement volume per well) Referred value indicates the average of multiple experiments and can differ for individual sample materials. The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode
- Maximum event rate: Up to 15.000 events/second
- Sample uptake volume: 1-450 µL
- Sample carry-over: 0.01 % Referred value indicates the average of multiple experiments and can differ for individual sample materials. For carryover, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 10,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 100 μL and measured at medium flow rate in standard mode. The carryover is defined by sum (CO-singlet count)/sum(SRC-singlet count) × 100%
- Flow rate: 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second
- Fluorescence performance: 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales
- Compensation: Automated, or manual with 8x8 matrix, during or after acquisition
- Operating system: Embedded operating system
- Software: MACSQuantify, integrated acquisition and analysis software
- Data files: .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)
- Components: Build-in PC, 15.6” LCD touchscreen, Robotic uptake unit for sample acquisition, integrated MACS magnetic cell enrichment unit
- Accessories:
- MACS® Chill Racks (5, 15, 50, 96)
- Universal Reagent Rack (130-115-722)
- MACSQuant® Buffer Supply Station (130-101-841)
- MACS® MiniSampler Plus (130-105-745)
- MACSQuant® Columns (130-094-458)
Brochures
Express Modes for MACSQuantify™ Software: Reliable Data at the Push of a Button
In this brochure, Miltenyi Biotec describes the unique add-on features for the MACSQuantify™ Software. Express modes are standardized data analysis tools that are optimized to automate flow cytometric measurements and analyses via predefined experiment settings, acquisition, and automated gating.
MACSQuant Flow Cytometers - Engineering the Future of Automation
The first MACSQuant® Analyzer was engineered to enable demanding and diverse flow cytometry applications of researchers around the world. At Miltenyi Biotec, we have since continued to expand our instrument portfolio to meet the evergrowing complexities in cell analysis. Innovation is at the core of each MACSQuant Instrument, offering automated flow cytometry solutions for a diverse array of appliations.
Wondering which MACSQuant Flow Cytometer is the best fit for your lab? Explore your options in the MACSQuant family of flow cytometers.
MACSQuant VYB - Instrument Specifications
The MACSQuant® VYB is a digital benchtop flow cytometer equipped with three lasers, 10 optical channels and intuitive software to facilitate rapid and simple cellular analysis. Configured with a 561 nm laser for enhanced analysis of fluorescent molecules in the yellow/green excitation range, the MACSQuant VYB is a versatile instrument for both phenotypic and functional analyses with fluorescent antibodies, dyes, and proteins. The flexible platform of the MACSQuant Instruments allows for analysis of many types of samples. The ACSQuant VYB is an excellent choice for researchers in the fields of immunology, systems biology, cell biology, neuroscience, cancer biology, and many other disciplines that require highly reliable and sensitive flow cytometric analyses.
Flow Cytometry Solutions - The MACSQuant VYB
The MACSQuant® VYB delivers all the performance, automation, compactness, and convenience of the MACSQuant Analyzer but with a uniquely configured optical layout, featuring violet, yellow, and blue lasers. With this new optical layout in combination with ten optical detection channels, the MACSQuant VYB is a powerful and versatile flow cytometer for virtually every lab.
Determination of Ploidy Levels in Plant Cells
Misdivisions during meiosis or mitosis can lead to a multiplication of the entire set of chromosomes. This phenomenon is called pluriploidy and occurs in many plants, in particular flowering plants (angiosperms). Plants can easily adapt to ploidy changes. Due to the changes, altered phenotypes can develop, and as a result the individuals may potentially occupy new ecological niches or outcompete species. Determination of the ploidy level is therefore a central aspect in many plant research fields, including taxonomy, plant development, and plant breeding.
Reliable, Hands-Free, and High-Throughput Cell Viability Assays on the MACSQuant Flow Cytometer
The determination of cell viability in any cell culture is a crucial analytical measurement for any study. This includeshigh-throughput cytotoxicity screens of large libraries of compounds and drug discovery research, and studies in all laboratories interested in validating their cell cultures upstream or downstream of any given application.
MACSQuant Flow Cytometers - FRET Express Mode
Fluorescence resonance energy transfer (FRET) is a mechanism describing an energy transfer between two fluorescent molecules. The efficiency of this energy transfer is inversely proportional to the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance. Measurements of FRET efficiency can be used to determine if two fluorochromes are within a certain distance of each other. Therefore FRET can be used to detect molecular interactions in a number of systems and has hence often been applied in various assays in biology and chemistry.
Rare Cell Isolation and RNA Amplification for Transcriptome Sequencing of Cancer Stem Cells
This application poster by Miltenyi Biotec combines cell enrichment, RNA amplification and high-throughput parallel sequencing to elucidate the specific gene expression profile of potential cancer stem cells, which are usually hidden by the bulk cell population. Isolation of CSCs from the tumor and amplification of tiny RNA amounts for transcriptome sequencing are addressed.
