MACSQuant® VYB Analyzer

Flow cytometry with the MACSQuant Family of flow cytometers is all about performance, automation, compactness, and convenience. The MACSQuant VYB not only combines these features, but also comes with a uniquely configured optical bench, featuring violet, yellow, and blue lasers. It is a perfect match for detecting fluorescent protein reporters or better discrimination between PE and FITC conjugates.

Technology highlight
Finding the right fit for fluorescent proteins with the yellow laser

• Fully utilize fluorescent proteins and reporter applications that require the Violet, Blue, or Yellow laser such as mCherry, GFP and CFP
• Optimal excitation of PE and PE tandem dyes
• Excite and analyse APC and APC tandems
• Minimize compensation for FITC/GFP and PE conjugates
• 21 CFR Part 11 compliant software option

Product Specs:

• Quantity: EA
• Dimensions: (WxDxH* *adjustable touch screen) 669x400x394-553.5mm
• Applications: Flow Cytometry
• Excitation:
  • 405nm (violet)
  • 488nm (blue)
  • 640nm (red)
• Detection: FSC, SSC, 8 fluorescence channels
• Regulatory status: For research use only
• Sample format: 1.5/2.0/5.0/15/50 mL tubes or 24/48/96-well plates
• Measurement speed: < 25 minutes per 96-well plate (5 µL measurement volume per well) Referred value indicates the average of multiple experiments and can differ for individual sample materials. The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode
• Maximum event rate: Up to 15.000 events/second
• Sample uptake volume: 1-450 µL
• Sample carry-over: 0.01 % Referred value indicates the average of multiple experiments and can differ for individual sample materials. For carryover, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 10,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 100 μL and measured at medium flow rate in standard mode. The carryover is defined by sum (CO-singlet count)/sum(SRC-singlet count) × 100%
• Flow rate: 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second
• Fluorescence performance: 5-decade logarithmic scale (10-2 to 10³), displayed in lin, log, or hlog scales
• Compensation: Automated, or manual with 8x8 matrix, during or after acquisition
• Operating system: Embedded operating system
• Software: MACSQuantify, integrated acquisition and analysis software
• Data files: .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)
• Components: Build-in PC, 15.6” LCD touchscreen, Robotic uptake unit for sample acquisition, integrated MACS magnetic cell enrichment unit
• Accessories:
  • MACS^® Chill Racks (5, 15, 50, 96)
  • Universal Reagent Rack (130-115-722)
  • MACSQuant^® Buffer Supply Station (130-101-841)
  • MACS^® MiniSampler Plus (130-105-745)
  • MACSQuant^® Columns (130-094-458)