Lactose Assay Kit
Sufficient for 100 colorimetric or fluorometric tests.
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Lactose is a disaccharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2–8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.
In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2–1.0 nmole lactose for the fluorometric assay and 2–10 nmoles lactose for the colorimetric assay.
