Human KRAS (2-185,G12C) Protein, His Tag (active enzyme)
Human KRAS (2-185,G12C), His Tag (KRS-H51H3) is expressed from E.coli cells.
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Synonyms
GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, KRAS2, RASK2, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, KRAS
Source
Human KRAS (2-185,G12C), His Tag (KRS-H51H3) is expressed from E.coli cells. It contains AA Thr 2 - Cys 185 (Accession # P01116-2(G12C)).
Predicted N-terminus: Met 1
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 23.0 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 500 mM NaCl, 1 mM PMSF, 2 mM TCEP, pH7.5. Normally trehalose is added as protectant before lyophilization.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
