Glucose-6-Phosphate Dehydrogenase Activity Assay Kit

Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
• Species reactivity- Mammalian
• Application- The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.

Features & Benefits:
• Simple procedure; takes ~ 30-60 minutes
• Fast and convenient

Kit components:
• G6PDH Assay Buffer
• G6PDH Substrate
• G6PDH Developer
• G6PDH Positive Control
• NADH Standard (0.5 µmol)

Description:
Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. BioVision's glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450nm. The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.