Fast-Link™ DNA Ligation Kit
Transform into E. coli without clean-up or desalting
cohesive end ligation
cohesive end ligation
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Applications
- Blunt-end and TA cloning of PCR products
- Ligation of next-gen sequencing adaptors to blunt-ended DNA
- Genomic DNA cloning and subcloning
- BAC/fosmid library construction
- cDNA cloning
- Linker ligation
The Fast-Link™ DNA Ligation Kit uses a high-quality ligase (Fast-Link T4 DNA Ligase), that was cloned at Epicentre and formulated to provide extremely rapid, high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. In contrast to other ligases, it is not necessary to desalt Fast-Link ligation reactions prior to the transformation of electrocompetent or chemically competent cells. The Fast-Link Kit can be used for routine and high-throughput DNA cloning.
Benefits
- Cohesive-end ligations in 5 minutes at room temperature
- Blunt-end ligations in 15 minutes at room temperature
- Ligation of PCR product with A-overhangs in 1 hour at 16°C
- High ligation efficiency
- Desalting of ligation products is not needed prior to the transformation of electrocompetent or chemically competent cells
- BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases
