EZ DNA Methylation-Lightning™ Kit
The EZ DNA Methylation-Lightning™ Kit features the fastest method for complete bisulfite conversion of DNA for methylation analysis.
EZ DNA Methylation-Lightning Bisulfite Conversion Kit
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Greats results.
DNA methylation
Easy-to-use kit. Quick results
Review Date: 9 Feb 2023 | Zymo Research
The EZ DNA Methylation-Lightning™ Kit features rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis.
Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
Breaking analytical barriers for fragmented samples: Revolutionizing FFPE samples in DNA methylation biomarker discovery
FFPE (Formalin-Fixed Paraffin-Embedded) tissue is a widely used sample type, as this tissue preparation method which involves tissue fixation, dehydration, clearing, and embedding, allows for long-term preservation of samples, maintenance of cellular morphology, and enabling of retrospective studies with archived samples. Samples have been stored in this form for decades and are essential in cancer research and diagnosis by providing a valuable resource for studying tumor biology, identifying potential therapeutic targets, and validating new diagnostic methods and treatments. In this application note, Zymo Research shows that while bisulfite converting FFPE DNA can be challenging due to the degraded nature of the sample, careful optimization of protocols and considerations for the compromised DNA quality can enable successful analysis of DNA methylation patterns in these valuable samples. Proper handling and analysis of bisulfite-converted FFPE DNA can provide critical insights into epigenetic changes associated with cancer and other diseases.
