EverBrite™ Hardset Mounting Medium with NucSpot® 640
Biotium’s hardset antifade mounting medium with NucSpot® 640 far-red nuclear counterstain.
Place your order directly with the manufacturer.
This antifade mounting medium features Biotium’s NucSpot® 640 nuclear stain, for one-step mounting and nuclear counterstaining.
Features
- Self-hardening antifade medium with refractive index well-matched to glass
- Featuring the only far-red nuclear counterstains available in mounting media
- Avoid cross-talk and photoconversion from DAPI
- Bright and specific nuclear staining with far-red NucSpot® 640
Spectral Properties
- NucSpot® 640: 649/668 nm (with DNA) (for Cy®5 channel)
EverBrite™ Hardset Mounting Medium cures to form a hard, permanent seal between the slide and coverslip, eliminating the need to seal the coverslip edges. It cures overnight to a refractive index of 1.42, and continues curing over the course of four days to a refractive index of 1.46. It prevents rapid photobleaching of a wide selection of fluorescent dyes. Unlike VECTASHIELD® Mounting Medium, EverBrite™ is compatible with cyanine-based fluorophores and is optimally formulated for use with Biotium’s CF® Dyes.
This mounting medium features Biotium’s novel NucSpot® 640 is a far-red dye nuclear counterstain for the Cy®5 channel. Using far-red nuclear stains can avoid problems of fluorescence cross-talk and UV photoconversion with traditional dyes like DAPI.
Note: NucSpot® 640 also shows dim blue fluorescence with the DAPI filter set, and should be tested for suitability before using with blue probes, especially for epifluorescence microscopy.
