DynaPro™ NanoStar™ II Dynamic Light Scattering Detector
The DynaPro™ NanoStar™ II DLS instrument determines size and molar mass with as little as 2 µL of solution.
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It gives an answer in a very short time with small amounts of samples.
characterize protein sizes
It is simple to use, doesn't require a lot of sample. It gives results in a very short time.
Review Date: 27 Mar 2023 | Waters
The DynaPro™ NanoStar™ II is a unique cuvette-based DLS instrument used for the analysis of proteins, gene therapy vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, liposomes, lipid, and metallic nanoparticles, and more. To accommodate scarce and precious samples, specially designed quartz cuvettes afford robust, accurate measurements with as little as 2 µL of solution, while our proprietary disposable cuvettes provide comparable sensitivity with as little as 4 µL
Walk-up operation with DYNAMICS® Touch™
The NanoStar is the only DLS instrument to provide a fully integrated, walk-up measurement experience. No need to boot up your lab PC, launch the DLS software, connect to the instrument and worry about where your data files are stored. DYNAMICS Touch is an intuitive—yet capable—DLS measurement app that resides right on the NanoStar. Via the full-size touch screen display, DYNAMICS Touch guides you through the entire setup and measurement process. It tells you if the data quality is sufficient for confidence in the results, and displays them instantly. Data files and concise PDF reports can be stored on the instrument, transferred via the network or downloaded to a flash drive. You can even send the report to your phone!
Features:
- Sensitivity: 0.1 mg/mL of 14.4 kDa lysozyme
- Hydrodynamic radius (Rh) range: 0.2 – 2500 nm
- Molar mass range: 300 Da – 1 MDa
- Temperature control: heated/cooled -10 to +120°C,
- Minimum sample requirement: 2 µL in quartz microcuvette, 4 µL in disposable microcuvette
- Optional infrared laser wavelength to eliminate fluorescence
- Can connect via optical fiber to a MALS detector for simultaneous online MALS and DLS measurement with size exclusion chromatography (SEC) or field-flow fractionation (FFF)
- Touch-panel display for immediate access to data, diagnostics and settings
- Software: DYNAMICS® analysis software offers comprehensive analysis of size and size distributions, molar mass, diffusion interaction parameter kD, second virial coefficient A2, thermal behavior Tagg/Tm/Tonset, DLS-based viscosity; full export of all results; optional 21CFR11 support for GMP
Applications:
- Size, aggregation and stability of small molecules, peptides, proteins, vesicles, nanoparticles, quantum dots and low/sub-micron colloids
- Protein quality control for interaction or structural studies
- Protein and polymer weight-average molecular weight
- Macromolecular interactions: diffusion interaction parameter kD and second virial coefficient A2 (B22)
- Thermal onset parameters Tagg, Tonset, Tm
- Solubility of polymers
- Critical micelle concentration and temperature
- Nanoparticle structure by combining MALS and DLS sizing
A biopharmaceutical workflow powered by Wyatt Technology
Reliable biophysical characterization is a cornerstone of biologics development, playing a critical role at every stage – from selecting promising drug candidates to optimizing formulations and ensuring consistent manufacturing processes. Explore a comprehensive guide on how to deliver biopharmaceutical solutions, beginning with light scattering technology.
Particle size analysis by DLS for optimization of protein complexes for measurement by small-angle neutron scattering
Explore how dynamic light scattering (DLS) can be used to ensure monodispersity in protein samples for small-angle neutron scattering (SANS) studies. Discover how researchers using the DynaPro™ Plate Reader, detected protein aggregation that was undetectable by SDS-PAGE and UV spectroscopy. This method enhances sample quality assessment, ensuring reliable SANS measurements for studying Type I bacterial restriction-modification systems.
Characterization of AAV-based viral vectors by DynaPro DLS/SLS instruments
In this application note, Wyatt Technology explores the utilization of the DynaPro Plate Reader and DynaPro NanoStar instruments for the quantification of three quality attributes (QAs) of AAV drug products: aggregate content, thermal stability, and total viral particle concentration. These two technologies, which combine dynamic light scattering (DLS) and static light scattering (SLS) respectively, represent low-volume, quick-screening tools for quantifying AAV quality attributes.
