Products & ReviewLife Sciences
DNase I
VWR International LtdAvailable: Worldwide
DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyses phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'- phosphate and 3'- OH groups.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Description
The enzyme activity is strictly dependent on Ca²+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg²+, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion. In the presence of Mn²+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt ends or with one or two nucleotide overhangs.
