Direct-zol™ RNA Miniprep Plus
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.
Direct-zol RNA Miniprep Plus Kit
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Great results
RNA extraction
The quality of RNA was good for sequencing.
Review Date: 5 Jun 2023 | Zymo Research
Sold me on the ease of use and time saving
Isolate RNA from human cell lines
I like the straightforward protocol direct from Trizol to column. Definitely faster than competitors (not quite 7 minutes though!). Clean high quality results, easiest for cell lines, primary cells gets trickier.
Review Date: 27 Mar 2023 | Zymo Research
Useful and effective for different type of samples.
RNA extraction from blood for RNA expression analysis.
Easy to use with a high yield of product. It includes a useful DNase step to get a RNA sample ready to downstream process like RT-PCR.
Review Date: 25 Mar 2023 | Zymo Research
Nice kit for viral detection
Viral RNA extraction from ear notch samples
Very nice kit with great RNA yield and purity.
Review Date: 10 Feb 2023 | Zymo Research
Great product and great outcomes. simple and easy to carry out. Highly recommended
high quality RNA was purified from the primary cells.
Total RNA, including small RNAs, is effectively isolated from the cells and tissues. The eluted RNA is of high quality and suitable for further molecular and gene expression assays.
Review Date: 8 Feb 2023 | Zymo Research
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).
Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA (see below).
The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
The entire procedure typically takes only 7 minutes.
MiniPrep Plus MiniPrep MicroPrep RNA Recovery 100 µg 50 µg 10 µg Minimum Elution 50 µl 25 µl 6 µl (Animal) Cells ≤ 107 ≤ 5 x 106 ≤ 106 Tissue ≤ 50 mg ≤ 25 mg ≤ 5 mg
*U.S. Patent No. 9,051,563 and other pending patents.
How to extract RNA from hard-to-lyse samples in TRIzol
RNA isolation is a challenging procedure. Unlike DNA, which is highly stable, RNA is very unstable, quickly digested by RNase enzymes if their activity isn’t immediately inhibited. One popular reagent in RNA isolation protocols is TRIzol®. TRIzol was specifically formulated to isolate total RNA from any sample type in the modern research laboratory, whether in academia or industry: cells, hard to lyse or solid tissue, and infectious biological samples. In this application, Zymo Research present the best RNA extraction methods for challenging sample types, such as hard-to-lyse samples.
