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Direct-zol™ RNA Miniprep

The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.

Zymo Research

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Average Rating 5.0

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RNA extraction

 

Average Rating 5.0

Application Area:

RNA extraction, cDNA sinthesis

The Kit for RNA extraction is easy to use, very fast, the amount of RNA is more than sufficient (~100 ng), not degraded!

Review Date: 25 Jul 2024 | Zymo Research

Will continue using for routine RNA prep since it is quite fast and easy

 

Average Rating 5.0

Application Area:

Isolate RNA for qPCR/gene expression analysis

Quick and easy to use. However, some difficult tissues/cells require extra steps and I'm still not sure how good it works for those (like PBMCs)

Review Date: 8 Feb 2023 | Zymo Research

Ready to use

 

Average Rating 5.0

Application Area:

RNA purification

It is easy to use by bypassing chloroform phase separation steps and provides purer RNA with min DNA contamination and with min loss

Review Date: 24 Jan 2023 | Zymo Research

The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar.

Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.

Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.

Features:

  • The entire procedure typically takes about 5-10 minutes
  • Quick, spin column purification of high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar
  • Bypasses phase separation and precipitation procedures
  • DNase I included for DNA-free RNA

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