CleanNGS RNA & DNA Purification & Size Selection
DNA and RNA clean-up for next generation sequencing library construction. 1ml sample is available by request.
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The CleanNGS kit offers a highly efficient magnetic bead based clean-up system for the purification of both DNA and/or RNA for next-generation sequencing workflows. CleanNGS provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).
Based on our proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.
Key Features:
- Designed for both DNA and RNA purification
- Ideal for (double-sided) size selection for Next-Generation Sequencing
- Efficient removal of unincorporated dNTPs, primers, primer dimers and other contaminants
- No centrifugation or filtration
Performance of CleanNGS in next-generation sequencing
Next generation sequencing (NGS) allows for the generation of robust information on the genetic diversity of organisms at the single cell, organ, species or population level. To perform an NGS experiment, users must prepare a sequencing library from a purified nucleic acid sample. Magnetic beads have many uses within NGS workflows. In addition to nucleic acid isolation and purification, magnetic beads are also used for size selection in NGS library preparation and library normalization. With emerging NGS technologies, there is an increased need for NGS library purification methods providing accurate results starting from low input amounts of DNA and/or RNA. Since CleanNGS (CleanNA) can be automated, as well as being used manually, it can be adopted in any NGS laboratory independent of sample throughput. In this poster, CleanNA compares the performance of CleanNGS in DNA cleanup versus two equivalent magnetic beads based kits.
Important milestone for further development
CleanNA obtains EN-ISO 13485 certificate
