Cancer Cell Line Medium XF
Xeno-free medium for the standardized in vitro cultivation of established cancer cell lines.
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The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.
Components:
The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium
Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).
Key features:
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Xeno-free and serum-free formulation
Standardized culture of cancer cell lines under serum and xeno-free condition
Traditional cancer cell culture methods often use fetal calf serum (FCS), which introduces variability and impacts research reliability. This application note describes the switch of an existing cancer cell culture to standardized Cancer Cell Line Medium XF - which offers a consistent, cost-effective, and xenofree environment - and subsequent routine culture.
Working with cancer stem cells
Cancer stem cells (CSCs) have an immense impact on cancer initiation, progression, metastasis, and relapse. Their ability to resist conventional therapies and drive tumor aggressiveness underscores the importance of understanding and targeting CSCs in the fight against cancer.
Download this comprehensive eBook: "Working with cancer stem cells", a complete guide on CSC biology, including an isolation protocol and the latest insights.
Cancer organoid model
In this application note, PromoCell describes a method for easy 3D organoid culture of Primary Human Dermal Fibroblasts (NHDF) and MCF- 7 cells in the Cancer Cell Line Medium XF.
