BS3 (bis(sulfosuccinimidyl)suberate), No-Weigh™ Format
Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
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BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
No-Weigh microtube handling:
Each of the eight microtubes contains 2 mg of crosslinker. Puncture foil with a pipette tip and add DMSO or DMF to the DSS or aqueous buffer to the BS3. Store the microtube strip in the foil pouch provided. Used microtubes may be cut from the unused microtubes and discarded.
