BrightStar™ ECL Detection kit, highly sensitive to Femtogram levels

BrightStar™ Femto Chemiluminescent Substrate Kit

• 100pg, 50pg, 25pg, 12.5pg, 6.25pg, 3.13pg • Block 5% Milk/TBST 1 hour • Incubate in conjugated antibody for 2 hours • Goat α-rabbit-HRP 1:10,000 in 5% milk/TBS-T (1/30/14)
• Wash 3x TBST 10 min • Incubate in substrate 5min, drain XS on filter paper

Immobilon lot 1331501 exp 08 JUL 2015
Pierce SuperSignal West Fempo lot OG186675
BrightStar™ 2 component lot 14029A/B 1/29/14
Lot 12249 4°C (From 9/7/12 Stability Assay)
Luminata Forte Lot 133055 exp 01 MAY 2015
BrightStar™ Lot 14029 1/29/14 single component

Expose on cooled CCD camera for 5 minutes at the indicated length of time after addition of the substrate • Analyze images on MutiGauge software for relative signal intensities with background correction