Biotage® Horizon 3100
Previously known as SPE-DEX ® 3100

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Great product, somewhat expensive. Good investment if you are performing > 300 samples per year.
Fats Oils and Grease analysis
Have used Biotage (formally Horizon) equipment for 20 years. Equipment is very reliable and long lasting with regular PMs.
Review Date: 17 Nov 2025 | Biotage
Manufacturer's Response
We sincerely appreciate your review and your long-term partnership over the past two decades. It’s great to hear that the Biotage® Horizon 3100 has remained consistently reliable with regular PMs. Your continued confidence in Biotage equipment means a great deal to us.
Oil and grease (hexane extractables) is a general indicator of the amount of hydrocarbon in a water supply. It is used around the world to indicate pollution and to help ensure reliable waste treatment plant operation.
In the past this measurement was often made by extracting the water with a chlorofluorocarbon solvent and infrared used for the measurement step. With concern about the ozone layer and important toxicity concerns, these solvents have been outlawed in many countries and n-hexane is now the extraction solvent of choice. A simple gravimetric measurement is used for the analytical step.
The Biotage® Horizon 3100 extractor, coupled with the safe solvent delivery system, Pacific Premium solid phase extraction disks, in-line extract drying, the Speed-Vap IV Evaporation system, and the SolventTrap og provides a full workflow process for oil & grease analysis.
Features:
- Fully compliant with regulatory methods such as US EPA method 1664B, ISO 11349, and Standard Methods 5520B.
- Uses the original sample bottle to dispense the sample and automatically rinses it, too.
- Simple to operate with touch-screen
- Controller and on-board methods.
- Disks and bottles of various sizes accommodated for best match with laboratory needs.
- Handles all types of samples with reliable operation.
- Bench-top design, frees up hood space.
Utilizing 250 mL sample volumes for EPA method 1664B extractions
EPA Method 1664B was developed for measuring oil and grease in wastewater with Hexane Extractable Material (HEM) concentrations ranging from 5 to 1000 mg/L. However, samples containing high oil and grease content (> 500 mg/L HEM) are challenging to extract and therefore the method allows for a smaller sample to be collected. To run reduced sample volumes, laboratories must be able to demonstrate the ability to meet the method detection limit (MDL) requirements at the reduced sample volume level. This application note provides a full solution for EPA method 1664B that meets the method detection limits at 250 mL sample volumes.


















