Azure Cielo 3 Real Time qPCR System
96-well Real-Time PCR instrument with 10.2” touchscreen interface, 3 dye channel filters
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The Azure Cielo 3 can bring you:
- Flexibility—Engineered for a wide variety of qPCR applications with high sensitivity and wide dynamic range
- Intelligent workflow—Our user interface allows simple touchscreen assay setup, and multi-user logins to make each run easy and reproducible
- Reliability—Our optics and thermal block are designed to deliver the same uniformity and reproducibility after 1000 qPCR experiments, giving you confidence in reliability of the machine
- Connectivity— no external PC is required to run the machine. Data can be transferred via Ethernet, LAN, Wi-Fi or USB
- Dye compatibility— SYBR Green/EvaGreen, FAM, VIC/JOE/HEX/CAL Fluor 540,
- CAL Fluor Orange 560, Cy5/LIZ/Mustang Purple
Brochures
Azure Cielo - Real-Time PCR
Explore this product brochure on the Azure Cielo™ to see all of the benefits of using the machine for high-performance real-time PCR.
Using the Azure Cielo Real-time PCR System for SARS-CoV-2 viral RNA detection
For early COVID-19 detection and diagnosis, real-time PCR instruments must be capable of measuring very small amounts of viral RNA. Researchers estimate the limit of detection (LOD), or the lowest concentration of RNA detected with > 95% confidence, can be as little as 1 copy of viral RNA per μL2. In this technical note, Azure Biosystems determine the LOD for the Azure Cielo™ using SARS-CoV-2 RNA.
Detection of low copy telomeric genes with the Azure Cielo
In this application note, the sensitivity of the Cielo is compared to that of a competitor instrument by comparing the Cq values observed with duplicate reactions run on the two instruments.
Azure Cielo: SNP Genotyping/Allelic discrimination
In this application note, Azure detail SNP genotyping using the Cielo qPCR instrument. They detect 2 SNPs using a commercially available kit. Using a 5’-nuclease allelic discrimination assay, they amplify the target from a DNA sample using a standard primer pair, followed by detection of each SNP with two differently labeled probes (FAM or VIC reporter molecule).
Azure Cielo: Adding dyes and Color Compensation
This application note explains how to add new dyes and describes how to run the Color Compensation Protocol to correct for potential crosstalk following a data run.
Azure Cielo Real-time PCR
In this video, Azure Biosystems introduces its Azure Cielo real-time PCR machine, designed to deliver the highest level of performance to bring out the best in your data. This promises a reliable system packaged with innovative and high-performance optical technology.
