Atlantis™ Columns
Most chromatographers have experienced problems retaining and separating polar compounds using conventional reversed-phase chromatography. These difficult-to-analyze compounds either pass through the column unretained or, if retained at all, co-elute at the beginning of the chromatogram. Waters AtlantisTM columns are designed for these types of challenging separations and are available in two chemistries: dC18 and HILIC sili…
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Most chromatographers have experienced problems retaining and separating polar compounds using conventional reversed-phase chromatography. These difficult-to-analyze compounds either pass through the column unretained or, if retained at all, co-elute at the beginning of the chromatogram. Waters AtlantisTM columns are designed for these types of challenging separations and are available in two chemistries: dC18 and HILIC silica. AtlantisTM dC18 columns are a fully LC/MS compatible line of difunctionally bonded C18 columns that exhibit superior retention of polar compounds under reversed-phase conditions. AtlantisTM HILIC silica columns are designed for high throughput LC/MS applications where the high organic mobile phase conditions used in hydrophilic interaction chromatography (HILIC) are desired. The use of HILIC mobile phases yields improved LC/ESI-MS response, direct compatibility with SPE solvents and complementary selectivity as compared to reversed-phase HPLC.
New Atlantis™ Intelligent Speed (IS) columns are powerful tools for the LC/MS scientist who is looking for faster methods and increased sample throughput.
Routine determination of trifluoroacetic acid (TFA) and difluoroacetic acid (DFA) in surface and drinking water by direct injection using UPLC-MS/MS
In this application note, Waters™ demonstrates a direct injection UPLC-MS/MS method for the determination of trifluoroacetic acid (TFA) and difluoroacetic acid (DFA) in surface and drinking water. The method performance study was completed on an ACQUITY UPLC™ I-Class PLUS System with a Xevo™ TQ-XS and electrospray ion source combination using a Waters Atlantis™ Premier BEH C18 AX Column. Samples were prepared by pipetting into a polypropylene injection vial and adding internal standard.
