ApopTag® Fluorescein In Situ Apoptosis Detection Kit
Detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that's conjugated to a Fluorescein reporter molecule
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy.
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit has been tested for specific staining in these model systems: (a) human normal peripheral blood lymphocytes induced with dexamethasone as stained in cytospins, (b) rat regressing mammary gland as stained in formalin-fixed, paraffin-embedded sections, and (c) human leukemic peripheral blood lymphocytes induced with camptothecin, as stained in cell suspensions and used for quantitative flow cytometry.
Components:
- Equilibration Buffer 90416 3.0 mL -15°C to -25°C
- Reaction Buffer 90417 2.0 mL -15°C to -25°C
- TdT Enzyme 90418 0.64 mL -15°C to -25°C
- Stop/Wash Buffer 90419 20 mL -15°C to -25°C
- Blocking Solution 90425 2.6 mL -15°C to -25°C
- Anti-Digoxigenin-Fluorescein 90426 2.1 mL 2°C to 8°C
- Plastic Coverslips 90421 100 ea. Room Temp.
- affinity purified sheep polyclonal antibody
