Ambion® Cells-to-CT™ Kits

Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection.

Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity, the kits contain reverse transcription (RT) reagents for cDNA synthesis and either probe-based (TaqMan® probes) or dye-based (SYBR® Green dye) master mixes for real-time PCR analysis.

The 1-step and 2-step kits differ in that the 1-step kits combine reverse transcription and real-time PCR into a single step, significantly reducing the number of pipetting steps and protocol time.

Features:

• Extraordinary ease and speed—96 samples for qRT-PCR in typically <10 min
• No tedious RNA purification—no columns, heating, or centrifugation
• Superior performance—designed for consistent accuracy, reproducibility, and sensitivity with 10–100,000 cells
• Validated kit workflows—complete sets of reagents preoptimized to work efficiently right out of the box

Available Kits:

• Cells-to-CT™ 1-Step TaqMan® Kit
• Cells-to-CT™ 1-Step Power SYBR® Green Kit
• TaqMan® Gene Expression Cells-to-CT™ Kit
• Power SYBR® Green Cells-to-CT™ Kit
• Single Cell-to-CT™ qRT-PCR Kit