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Aeris Core-Shell LC Columns for Proteins and Peptides

Core-Shell Particles Precision Engineered for Protein and Peptide Separations.   Core-shell particle technology provides striking increases in peak capacity and resolution at lower backpressure, giving chromatographers the ability to achieve ultra-high performance on ANY system, HPLC or UHPLC. A uniform porous silica layer is grown around a solid, spherical silica core, providing effective retention and selectivi…

Phenomenex Inc

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Average Rating 4.3

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Really useful column for peptide separation

 

Average Rating 4.3

Application Area:

Analysing protein samples with HPLC

We found the Aeris column very effective for analysis of our multi-peptide samples by HPLC. Really good separation of a fairly complex mixtures. The results were very robust and reproducible and the after sales care was also great, to help us optimise our experiments.

Review Date: 4 Sept 2023 | Phenomenex Inc

Core-Shell Particles Precision Engineered for Protein and Peptide Separations.

Core-shell particle technology provides striking increases in peak capacity and resolution at lower backpressure, giving chromatographers the ability to achieve ultra-high performance on ANY system, HPLC or UHPLC.

A uniform porous silica layer is grown around a solid, spherical silica core, providing effective retention and selectivity with improved resolution, speed, and recovery. Next, optimizing the pore size and shell thickness for intact proteins or smaller peptide fragments provides well-defined depth penetration of biomolecules leading to maximum separation power.


Column Specifications:

  • Phase: PEPTIDE XB-C18, WIDEPORE C4, WIDEPORE XB-C18, WIDEPORE XB-C8
  • Particle Size: 1.7, 2.6, 3.6, 5.0 µm
  • Pore Size: 200, 100 Å
  • Length: 50, 100, 150, 250 mm
  • ID: 2.1, 3.0, 4.6, 10.0, 21.2

PEPTIDE XB-C18

  • Synthetic peptide impurity analysis
  • Peptide mapping
  • Identifying protein modifications
    - Glycosylation
    - Substitution
    - Truncation
  • Analyzing post-translational modifications
    - Deamidation
    - Oxidation
    - Deletions

WIDEPORE XB-C18

  • Proteins
  • Hydrophilic proteins
  • PEGylated proteins
  • High temperature separations
  • Alternative selectivity for peptide mapping

WIDEPORE XB-C8

  • Large proteins
  • Moderately hydrophobic proteins
  • Monoclonal antibodies
  • Glycosylated proteins
  • High temperature separations

WIDEPORE C4

  • Very large proteins
  • Very hydrophobic proteins
  • Membrane proteins
  • Least retentive

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