2-Mercaptoethanol
For molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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Application
Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
- as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
- to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
- to trypsinize mouse embryonic stem cells
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
