Wyatt Technology’s new DynaPro plate reader enables rapid buffer optimization

Wyatt Technology Corporation has extended the capabilities of its innovative DynaPro™ Titan Plate Reader system to identify the optimal buffer conditions for protein purification and concentration.
Buffer optimization is of enormous commercial interest for the drug discovery process as it affects the success of High Throughput Screening and biophysical characterization studies.

Identifying optimal buffer conditions for protein purification and concentration is laborious and time consuming as there are a large number of buffer conditions to be examined. Wyatt’s groundbreaking DynaPro™ Titan Plate Reader drastically increases throughput, enabling scientists to overcome such problems and effectively characterize the homogeneity of a protein in solution.

The new DynaPro™ Titan Plate Reader is a stable, automated Dynamic Light Scattering (DLS) system that achieves superior performance and increased productivity with fewer than 50 microliters of sample per well (recoverable). This revolutionary instrument utilizes state-of-the-art technology and is equipped with industry standard microplates (96, 384 or 1536 well plates). Being non-invasive and filter-free, this new product is particularly easy to use and can be customized to suit all analytical needs. In the 1536 well plate mode, the amount of sample required is less than 5 microliters.

A new application reveals that with the help of an optimally designed buffer screen, the DynaPro™ Titan Plate Reader is now able to identify the factors or interaction of factors important for protein homogeneity more easily. Results of the application demonstrate that the utilization of Wyatt’s DynaPro™ Plate Reader ensures excellent data reproducibility and massive savings in time. In the buffer screen, the Auto Laser Power Adjust function available on the DynaPro™ plate reader provided the optimal measuring conditions for every well. The program returned 72 different buffer conditions to be screened compared to about 400 buffers in the case when one separate factor is changed at a time.

The DynaPro™ Titan Plate Reader is capable of performing a vast range of applications quickly and easily, including bioprocessing and quality control of viral particles and vaccines, screening chemical compounds for small molecule aggregation or potential promiscuous inhibitors and exploring antibody formulations over a broad range of solution conditions.