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Wyatt Technology’s Eclipse significantly improves resolution for protein and particle separation

2 Nov 2006

Product news

Wyatt Technology, the world leader in absolute macromolecular characterization instruments and software, introduces its Eclipse separation system.

Designed for the analysis of protein, biopolymer and colloidal materials, the Eclipse system has been developed as an alternative to conventional column chromatography and is based on asymmetrical Flow Field-Flow Fractionation (AF4). Samples can be separated according to hydrodynamic radius in a selected channel. Wyatt Technology’s new Eclipse channel technology significantly improves resolution whilst increasing peak height in the detector, without compromising resolution or peak shape.

The Eclipse is the only AF4 system that requires only one pump to generate all three flow rates that are required for the analysis. Additionally, the absence of pulsations is even more vital when the channel volume is reduced. Developed for the pharmaceutical industry, the Eclipse is able to characterize antibody preparations with respect to aggregation and allows for Impurities or contaminants, like aggregates, to be identified at a lower detection limit, as well as featuring a reduced dead volume.

Separation is achieved by interacting with a cross-flow permeating through the bottom wall, while the sample is transported by the longitudinal flow towards the channel outlet and the detectors. Endorsed by the Food and Drug Administration (FDA) as a necessary check of Size Exclusion Chromatography (SEC) analysis, there is no stationary phase which would result in the absorption or degradation, thereby enabling the separation of soluble molecules from colloidal constituents.

As well as enhanced resolution and peak height, consumption of eluent and analysis time is also reduced. The smaller channel is easier to handle for membrane replacement and has faster equilibration time, resulting in less down time and increased sample throughput.

Coupled with the new generation of Wyatt Technology’s MALS detectors, the HELEOS and TREOS, characterization of proteins and biopolymers at nano-gram concentration range is feasible.

Tags

Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Wyatt Technology’s Eclipse significantly improves resolution for protein and particle separation