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Win 1 of 3 iPad Minis at the Mirus Bio Booth #423 at ASCB

Mirus Bio LLC
3 Dec 2012

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Stop by and tell us about your research! Find out about how toxicity can affect the efficiency of your transfections, the latest transfection applications for stem cell research, or to sign up for an iPad mini.

Since 1995, scientists have relied on Mirus Bio’s high efficiency, low toxicity TransIT®- Transfection Reagents. We offer broad spectrum or cell line specific delivery solutions that are ideal for routine use as well as specialized applications, such as stem cell generation, viral production and protein expression.

Also, stop in for three information-packed seminars:

Sunday, Dec 16, 1:45-2:45, Room 105
Human Induced Pluripotent Stem Cells: Their Use in Basic Research, Drug Discovery, and Toxicity Testing
presented by Blake Anson, Ph.D., Cellular Dynamics International

The primary strength of human induced pluripotent stem cell (iPSC) technology is that it brings relevant human biology into the laboratory setting. This technology will further progress our understanding of relevant biological mechanisms and empower the translation of advances in basic research to implementation in drug discovery and regenerative medicine. This presentation will discuss advances in iPSC technology, provide functional characterization data on a variety of iPSC-derived tissue cells including cardiomyocytes and neurons, and demonstrate the utility of these human cells across a variety of functional and investigative methodologies for use in applied and basic research.

Sunday, Dec 16, 2:45-3:45, Room 105
Transfecting Stem Cells – Why, Where and How?
presented by Miguel Dominguez, M.S.

Stem cells hold the promise of revolutionizing therapy for a myriad of diseases. Researchers can utilize stem cells for elucidating disease models through differentiation of normal/diseased cells into relevant and homogenous cellular lineages. Transfection of nucleic acids such as plasmid DNA, mRNA and siRNA serve as a vital tool in stem cell reprogramming and differentiation processes. Once reprogrammed, the induced pluripotent stem cells (iPSC) can be used to derive a multitude of cell types, including cardiomyocytes and neurons. Purity of stem cell derivatives can be augmented by promoter-specific selection achieved via transfection. These validated cell populations represent an untapped resource for biological research. This showcase will illustrate the recent applications and importance of high efficiency and low-toxicity transfection in the stem cell arena.

Monday, Dec 17, 6:45-8:15, Room 111
Transfection Fundamentals: Secrets to Success
presented by Anjana Bhattacharya, Ph.D.

Delivery of nucleic acids such as plasmid DNA, siRNA, and mRNA into cells using chemical transfection or electroporation is pivotal to current cell biology research. Proper choice of the transfection technique and optimization of key experimental factors is imperative when working with physiologically relevant cell types that are hard-to-transfect, e.g. stem cells and primary cells. In this tutorial, we will outline new and current transfection tools including their mechanism, efficiency and cytotoxicity potential. We will discuss direct labeling of nucleic acids for tracking and visualizing nucleic acid delivery and share helpful tips for assessing and optimizing transfection output. Furthermore, to address transfections in a more biologically relevant context, we will present a novel 3D scaffold approach to more accurately mimic the in vivo tissue environment.

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TransIT®-2020 Transfection Reagent

Mirus Bio LLC

A high performance, animal-free, broad spectrum DNA transfection reagent. Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cell lines and primary cells Outperforms Competitor Reagents - TransIT®-2020 demonstrated higher protein yield when compared to FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD Superior Transfection of Insect Cells - Obtain higher expression than other insect cell transfection reagents Animal Origin Free - TransIT®-2020 provides high performance with maximum compatibility  

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TransIT®-LT1 Transfection Reagent

Mirus Bio LLC

TransIT®-LT1 Transfection Reagent from Mirus Bio- A broad spectrum, low toxicity, DNA transfection reagent • Broad Spectrum DNA Delivery - Utilize one transfection reagent and protocol for a variety of cells. • Low Cellular Toxicity - Maintain cell density and reduce experimental biases. • High Efficiency Delivery - Achieve expression in a large population of cells for experimental success. • Deliver Single or Multiple Plasmids - Suitable for many applications such as gene expression, shRNA expression, viral production, and promoter analysis.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Compound LibrariesCompound libraries, or chemical libraries, are used in drug discovery for the identification of potential therapeutics compounds. Used in conjunction with high-throughput screening, the libraries of stored compounds are often generated for specific purposes as a drug target or disease model. Cheminformatics are commonly used when designing a compound library and software can be used to analyze the screening process.  Medicinal ChemistryMedicinal chemistry is a broad discipline encompassing the design, identification, synthesis and development of chemicals in drug discovery. It includes a number of techniques covering structural biology, synthetic chemistry and molecular biology. Technologies used in medicinal chemistry include ADME, lab-on-a-chip, high content screening and assay assembly.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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Win 1 of 3 iPad Minis at the Mirus Bio Booth #423 at ASCB