Wave Goodbye to Time-Consuming Large Format Vertical Gel Electrophoresis

8 Oct 2012

Product news

Helping to make life that 'little bit easier in the Lab' is one of the objectives that drives Gel Electrophoresis specialists Cleaver Scientific (CSL) to continually develop new products which save time and space. A good example of this is the new VS20 Wave Maxi System - their latest innovation for large-format vertical gel electrophoresis.

The VS20 Wave is designed to perform a variety of separations, ranging from first and second dimension SDS-PAGE to capillary tube gel IEF and electroblotting. However, unlike other systems whose traditional clamping configurations require adjustment of as many as 24 screws to secure just two glass plates, the VS20 WAVE insert utilizes only four screws to secure as many 20 x 20cm gels. This innovative vertical screw-clamp technology ensures not only much faster set-up speed it also exerts uniform pressure along the height of the glass plates to facilitate a leak-free seal without gel compression and bowing to provide even migration.

The ergonomic WAVE-like design of the PAGE insert aids both handling and set-up, while its built-in inner buffer chamber allows set-up to be completed without the inclusion of a top tank or upper buffer chamber. A detachable cooling coil is supplied enabling the WAVE to be connected to a re-circulating chiller for uniform, trouble-free electrophoresis and this also permits runs to be performed faster at higher voltage.

The WAVE also benefits from a high-specification injection-molded construction, while its modular design and optional conversion kit allow blotting and isoelectric focusing techniques to be undertaken in the same all-purpose tank.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Isoelectric FocusingElectroblotting
Wave Goodbye to Time-Consuming Large Format Vertical Gel Electrophoresis