Waters Corporation Introduces New Line of Peptide Separation Columns

Waters Corporation has introduced new columns for peptide analysis and isolation based on its proprietary C18 BEH Technology™ particle platform.

The new Peptide Separation Technology columns are available in a 1.7µm – 10µm particle size range, lengths from 50 to 250mm and internal diameters (ID) of 75µm to 30mm. Waters is taking orders for custom prepared columns as well. Nominal particle pore sizes are either 130 or 300Å pore sizes, with plans to deliver the larger 300Å pore size columns in November of ’06.

These columns are intended for proteomics applications, peptide mapping of biopharmaceuticals, and for the characterization and purification of synthetic peptides.

The new Peptide Separation technology columns, in combination with all Waters HPLC Systems, ACQUITY UPLC™ and nanoACQUITY UPLC™ instruments, the columns offer scientists many benefits including improved separation of protein digests, significantly improved peptide mapping, efficient glycopeptide separations, and ability to quantify trace peptide variants in peptide mapping.

Specifically the Peptide Technology Separation columns improve the chromatography of peptides by offering scientists narrow and symmetrical peaks for best resolution; broad applicability by separating a wide range of peptides, both large and small, acidic and basic, hydrophobic and hydrophilic; and excellent peak shape and retention in formic acid and trifluoroacetic acid.

Peptide Separation Technology columns are integral elements of Waters Assured Performance Solutions; a combination of instrumentation, chemistry, and software optimized for particular applications. More information on these columns is availableon the article webpage.

Peptides are short sequences of amino acids derived from proteins usually by enzymatic digestion. By mapping, or separating and quantifying, individual peptides, life scientists can learn a significant amount about the identity and structure of specific protein molecules like human insulin. Central to successful peptide analysis are tools like liquid chromatography – often in combination with mass spectrometry – and analytical columns and chemistries specifically designed for peptide applications.