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Waters ACQUITY UPLC with 2D Technology Tackles Complex Separations Challenges

Waters
15 Mar 2011
bridget bridget
Laboratory Director

Product news

Waters Corporation announced today the addition of Waters® ACQUITY® UPLC System with 2D Technology to its growing portfolio of ACQUITY UPLC® systems. Designed for chromatographers, 2D Technology for Waters ACQUITY UPLC Systems gives separations scientists added resolving power, particularly for samples that are hard to resolve by conventional means, while offering greater sensitivity and selectivity.

Based on Waters proven ACQUITY UPLC technology, the Waters ACQUITY UPLC System with 2D Technology gives scientists expert capability for trapping, heart cutting and parallel column regeneration to eliminate matrix effects, increase overall speed of analysis, enhance assay ruggedness, increase selectivity and sensitivity and use MS-incompatible solvents for the first dimension of separation.

PPD, Inc. (Richmond, VA) routinely employs two-dimensional chromatography for separations demanding extra speed, separations power, and sensitivity. “We have a total of 54 LC/MS systems and most are set up to run 2D chromatography. Eighty percent of the time, our scientists use a simple 2D approach to remove residual lipids from the sample matrix”, says Rand Jenkins, Scientific Director of Chromatography Sciences at PPD.

For more demanding assays requiring maximum selectivity and sensitivity, PPD scientists employ 2D heart-cutting techniques using two analytical columns to isolate the analyte peaks of interest. “The ability to perform two dimensional UPLC separations using Waters technology has enabled some of our most challenging assays that require ultra-high sensitivity while maintaining excellent ruggedness and instrument up-time“, says Jenkins.

The Waters ACQUITY® UPLC System with 2D Technology features MassLynx Software for operational control and data management. It is a compatible front-end to Waters Synapt® and Xevo® brand mass spectrometers and quadrupole mass detectors.

Waters is offering its present ACQUITY UPLC customers a path to upgrade their systems to enable 2D separations with the addition of several hardware components and new software.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.

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Waters ACQUITY UPLC with 2D Technology Tackles Complex Separations Challenges