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Visit R&D Systems at AACR to View the New Microplate-based Antibody Array

Bio-Techne
11 Apr 2010

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R&D Systems presents the Proteome Profiler™ 96Microplate-based Antibody Array. Utilizing expertise in antibody and ELISA technologies, the Proteome Profiler™ 96 Microplate-based Antibody Array aims to make multi-protein detection more efficient than ever. The assay relies on a 96-well microplate that is pre-spotted with a carefully selected panel of capture antibodies in each well. When experimental samples are added to the wells, up to 16 different proteins can be detected simultaneously in a single sample using a two-site sandwich immunoassay.

Proteome Profiler 96 Microplate-based Antibody Arrays offer the advantages of multianalyte profiling in a format amenable to high-throughput analysis. They allow data to be collected in approximately 3.5 hours, utilize small sample sizes (50-100 μL), and can identify proteins of interest without having to perform multiple immunoprecipitation/ Western blot experiments.

For more information, please follow the link to the company article page at the top right of this page.

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Proteome Profiler Antibody Arrays

Bio-Techne

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays include:R&D Systems Human Phospho-RTK Antibody Array (Catalog # ARY001)Detect the relative site-specific phosphorylation levels of 42 different receptor tyrosine kinases(RTKs). R&D Systems Human Phospho-MAPK Antibody Array (Catalog # ARY002) Detect the relative site-specific phosphorylation levels of 19 different intracellular serine/threonine kinases. R&D Systems Human Phospho-Kinase Antibody Array (Catalog # ARY003) Detect the relative phosphorylation levels of 46 intracellular serine/threonine/tyrosine kinases. R&D Systems Human Phospho-Immunoreceptor Antibody Array (Catalog # ARY004) Detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors. R&D Systems Human Cytokine Antibody Array, Panel A (Catalog # ARY005) Detect the relative levels of 36 cytokines, chemokines, and acute phase proteins simultaneously. R&D Systems Mouse Cytokine Antibody Array, Panel A (Catalog # ARY006) Detect the relative levels of 40 cytokines, chemokines, and more simultaneously. R&D Systems Human Angiogenesis Antibody Array (Catalog # ARY007) Detect the relative levels of 55 angiogenesis-related proteins simultaneously. R&D Systems Rat Cytokine Antibody Array (Catalog # ARY008) Detect the relative levels of 29 cytokines, chemokines and more simultaneously. R&D Systems Human Apoptosis Antibody Array (Catalog # ARY009) Detect the relative levels of 35 apoptosis-related proteins simultaneously. R&D Systems Human Pluripotent Stem Cell Antibody Array (Catalog # ARY010) Detect the relative levels of 15 pluripotent stem cell markers simultaneously.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Visit R&D Systems at AACR to View the New Microplate-based Antibody Array