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Versatile high-resolution mass spectrometer expands Thermo Scientific Orbitrap platform

Thermo Fisher Scientific

Thermo Scientific Orbitrap Exploris 240 mass spectrometer drives discovery and identification with quantitative precision and accuracy to meet customers' specific analytical requirements

28 May 2020
Diane Li
Assistant Editor

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A new generation, high resolution mass spectrometer (MS) is designed to give proteomics, metabolomics, biopharmaceutical characterization and small-molecule scientists the analytical performance required for research and high-throughput analyses. The new system will help drive discovery and identification with increased accuracy for confident scale-up while operational simplicity and speed streamline time to results.

The Thermo Scientific Orbitrap Exploris 240 mass spectrometer expands the proven Orbitrap Exploris platform and delivers mass accuracy, sensitivity and resolving power across a wide dynamic range. With new-generation system architecture and instrument control software, the system provides simple yet powerful data acquisition capabilities, addressing the most demanding analytical challenges for small- and large-molecule applications. The system offers positive/negative mode switching for comprehensive sample coverage, fast scan speeds and functionalities including Thermo Scientific AcquireX intelligent data acquisition workflow that enable greater automation.

"Scientists are seeking technologies that can streamline and speed proteomics studies to clinical applications, reduce time to market for candidate biotherapeutics and address the growing challenge of characterizing novel chemical entities in small-molecule studies," said Iain Mylchreest, vice president, research and development, analytical instruments, Thermo Fisher Scientific. "As part of our expanded portfolio of new-generation instruments, the Orbitrap Exploris 240 mass spectrometer delivers the high performance and versatility needed to drive productivity across multiple applications."

Dr. Timothy Garrett, associate professor, department of pathology, immunology and laboratory medicine, co-director of the Southeast Center for Integrated Metabolomics, University of Florida, said, "Using the Orbitrap Exploris 240 mass spectrometer has been extremely user friendly, with one-click calibration and method templates for fast instrument setup. The system reproducibility in mass accuracy and peak integrated area over several sample batches and several days of acquisition has been impressive. This is very important for our large-scale metabolomics projects enabling minimum post-acquisition data manipulation to yield high quality results."

The Thermo Scientific Orbitrap Exploris portfolio of high-resolution, accurate-mass (HRAM) systems, which launched in 2019 with the Thermo Scientific Orbitrap Exploris 480 mass spectrometer, now includes two new, highly capable instruments, the Thermo Scientific Orbitrap Exploris 240 and Thermo Scientific Orbitrap Exploris 120 mass spectrometer, allowing customers to easily transfer knowledge and methods from research and discovery to routine testing. The Orbitrap Exploris 480 and 240 mass spectrometers are fully compatible with the Thermo Scientific FAIMS Pro interface to achieve enhanced identification of proteins and peptides. Improved quantitative performance for translational proteomics and high-throughput studies will enable researchers to accelerate the translation of discoveries into clinical applications.

Dr. Jonathan Bones, principal investigator, National Institute for Bioprocessing Research and Training, said, "The Orbitrap Exploris 240 mass spectrometer is a reliable and flexible solution for high-performance biopharmaceutical characterization and achieves impressive sensitivity for native MS analyses. With the Thermo Scientific Vanquish Duo UHPLC systems running in tandem mode, there is no more waiting during column equilibration; the mass spectrometer constantly acquires valuable data with all the available features of the Thermo Scientific Chromeleon Chromatography Data System (CDS) software. The system is a must-have instrument for analytical and characterization laboratories supporting biopharmaceutical development and manufacture."

Performance of the Orbitrap Exploris 240 mass spectrometer is further enhanced with access to powerful new software functionality to improve data acquisition and processing:

  • Proteomics scientists will benefit from the latest artificial intelligence (AI) technology with Thermo Scientific Proteome Discoverer 2.5 software. The new release utilizes deep learning tools to provide a more accurate prediction of mass spectra, improving confidence in peptide identification.
  • Supporting scientists developing new biopharmaceuticals, Thermo Scientific BioPharma Finder 4.0 software delivers enhanced flexibility for the analysis of intact proteins, sub-unit mass analysis, peptide mapping and oligonucleotide analysis. Direct transfer of data processing parameters to Thermo Scientific Chromeleon Chromatography Data System (CDS) software enables compliance-ready analysis for peptide monitoring and quantitation of proteins.
  • With Thermo Scientific Compound Discoverer 3.2 software, small-molecule researchers are supported through extensive characterization capabilities, access to multiple mass spectral fragmentation library and structural database sources. Access to the large Thermo Scientific mzCloud mass spectral fragmentation library enhances connectivity and characterization for small molecule applications to help identify compounds.
  • The launch of Thermo Scientific Xcalibur 4.4 software brings the AcquireX intelligent data acquisition workflow to the Orbitrap Exploris family of mass spectrometers, allowing greater automation for structural identification and characterization of small molecules.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Combinatorial ChemistryCombinatorial chemistry, also known as combichem, is a technique used in drug discovery to create libraries of structurally related compounds. A library is generated by synthesis with a chemical reactor system or by computer-based modeling of compound combinations. When undertaking combinatorial chemistry consider reagents, buffers, resins and standards.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Clinical MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Development SoftwareComputational techniques used in both the chemistry and biology aspects of drug development, for data acquisition, data analysis, processing and storage. Software is used for analysis of ADME results, toxicology, clinical trials and regulatory processes. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.ImmunologyImmunology is the branch of medical science that covers the study of all aspects of the immune system of multicellular organisms.Digital PathologyDigital pathology involves the use of digital imaging and computational tools to analyze pathology slides. It is transforming diagnostics, research, and education by offering enhanced image storage and analysis. Explore digital pathology solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Data AnalysisThe analysis of data is the process of transforming, modeling and evaluating data to discover useful information from experimental results.

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Versatile high-resolution mass spectrometer expands Thermo Scientific Orbitrap platform