University of York integrate Nonlinear’s SameSpots for a new approach to 2D image analysis

8 May 2006
Kerry Parker
CEO

Product news

Nonlinear Dynamics Ltd, a leading global provider of bioinformatics solutions, today announced that The Department of Biology of the University of York has adopted Nonlinear’s SameSpots approach as part of their 2D proteomics workflow. Under the purchase agreement, The Technology Facility within the Department of Biology has licensed TT900 S2S and have upgraded their existing 2D analysis software to Progenesis PG240 to support this new approach.

This purchase represents the latest key sale of products to enable the SameSpots approach following its official release at US HUPO in March. To date, over ten institutions across Europe and the US have adopted SameSpots into their 2D workflow. This impressive uptake by proteomics researchers reflects the significant benefits users can achieve with SameSpots, including 100% matching across all images and no missing values within the 2D data.

Dr Peter Ashton of The Technology Facility within the Department of Biology at the University of York, said, “Since implementing the SameSpots approach, the changes we have noted in our analysis have been very encouraging. The ability to perfectly align images within the experiment before applying SameSpots detection dramatically improves matching performance. This enables highly consistent and reliable results to be obtained from all our 2D data sets, even those that previous image analysis packages were unable to accurately quantify.”

Will Dracup, Group CEO of Nonlinear Dynamics, said, “The University of York have been valued customers for some years now and we are pleased to continue this relationship with their latest purchase. The statistical power of the SameSpots approach enables users to instantly enhance their 2D data and overcome many existing gel processing issues to obtain highly objective and accurate image analysis results.”

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
University of York integrate Nonlinear’s SameSpots for a new approach to 2D image analysis