Two-Color Protein Marker Offers Grayscale and Pseudo-Colored Images For Publishing

23 Feb 2012

Product news

LI-COR® Biosciences introduces the IRDye® (680/800) Protein Marker that is visible in the 700 nm and 800 nm channels of fluorescent imaging systems, such as the Odyssey® family of imaging systems. Grayscale or pseudocolor images of the IRDye Two-Color Marker can be viewed, exported, and used for publishing with both channels visible.

This two-color marker is designed to provide a ladder of convenient and consistent protein sizes (7.5 – 250 kDa) for use with polyacrylamide gels on Western blot membranes. In blot applications, the IRDye (680/800) Protein Marker can be used as a reference for lane or sample orientation.

“The new IRDye two-color protein marker is supplied ready-to-use and contains 12 proteins ranging in size from 7.5 – 250 kDa,” says Shawn Mischnick, LI-COR technical product specialist. “This marker adds increased performance for researchers who need to show both channels in a grayscale image”

Dependent on load volume, this marker can act in a variety of ways: as a pre-stained marker in gels, to visualize the progress of protein separation during electrophoresis. Or to determine the molecular weight of unknown proteins. To learn more about IRDye infrared dye reagents, visit the company article page.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Infrared ImagingIrdyeFluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.
Two-Color Protein Marker Offers Grayscale and Pseudo-Colored Images For Publishing