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Two at a time: Immunoblot Myositis plus

ORGENTEC Diagnostika GmbH
7 Aug 2008

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Myositis plus benefits its users in several aspects: The novel immunoblot by ORGENTEC Diagnostika not only facilitates the certain interpretation of a cytoplasmic immunofluorescence pattern and the correlation with antibodies relevant for diagnosing autoimmune disorders. In addition to that this new type of immunoblot allows for distinguishing antibodies related to myositis from those antibodies specific for this autoimmune disease. By this means this immunoblot is predestinated to support diagnosis of autoimmune myositis.

Provided with nine antigens, Myositis plus is a high-performance test system for clinical diagnostics of several autoimmune disorders, such as idiopathic myositis, polymyositis/scleroderma overlap syndromes, primary biliary cirrhosis (PBC), antisyntethase syndrome, or systemic lupus erythematosus. The antigens applied on Myositis plus are: M2, Jo-1, PM-Scl-100, PL 7, PL 12, Mi-2, Ku (p70/80), SRP, Rib-P.

Noteworthy is the SRP complex (signal recognition particle) that in this innovative immunoblot system is used together with all the six polypeptides and the 7SL RNA, in contrast to other test systems. By the same token, the Ku (p70/80) antigen, which is utilised in its native and non-denaturated form, stands alone.

Myositis plus is available in two packages sizes, 8 and 16 immunoblot strips; all reagents are ready to use. As well as all immunoblots by ORGENTEC Diagnostika, each strip is provided with a cut-off control, a serum and a conjugate control, and features an individual coding that prevents interchange of tests.

The immunoblot technology is particularly valuable to differentiate autoantibody profiles after ELISA screening or immunofluorescence tests. They are fast to be carried out, economically priced, easy to interpret, and they pay off quickly.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.

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Two at a time: Immunoblot Myositis plus