TwistDx Launch Brand New Liquid-Based Isothermal Designed to Entirely Replace PCR Reagents

6 Jul 2017
Abigail Berry
Administrator / Office Personnel

Product news

TwistDx have launched a new range of liquid format RPA kits that are ideal for high throughput laboratory use where DNA/RNA amplification is needed within 15 minutes without a thermocycler, and for researchers who want the flexibility to experiment with their RPA assay configurations.

The TwistAmp® Liquid kits provide RPA assay reagents in a glycerol-stabilized, liquid format that will be familiar to anyone who carries out PCR and other enzyme-based reactions in the laboratory. The reagents are provided in separate tubes, and users can pipette out as much or as little of the enzyme mix as they want to make up RPA reaction volumes of their choosing, create a bulk master mix as the foundation for multiple reactions, or experiment with ratios of RPA components to optimize their assays.

The new TwistAmp® Liquid kits offer an alternative format to TwistDx's flagship ambient temperature-stable lyophilized TwistAmp® RPA kits, which are ideal for the development of field and site-based assays. The liquid format will make it easy for laboratories to use RPA routinely as a fast, user-friendly alternative to PCR, sometimes even using the same PCR primers, and to develop customized assays.

The new TwistAmp® Liquid range includes TwistAmp® Liquid Basic, TwistAmp® Liquid Basic RT, TwistAmp® Liquid exo, and TwistAmp® Liquid exo RT.

Find more information about TwistDx products here.

Links

Tags

High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.