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TriStar²S LB 942 with HTRF®-Technology

BERTHOLD TECHNOLOGIES GmbH & Co. KG
15 Sept 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

The successful Berthold Technologies multilabel reader TriStar2S now takes the next step. Right after the recent introduction of the monochromator technology, the TriStar2S now also supports time resolved fluorescence energy transfer assays (TR-FRET, HTRF®). FRET is an excellent technology to monitor specific molecular interactions with high sensitivity in homogeneous phase.

The effect relies on the distance-dependent transfer of the excitation energy from a donor-dye to an acceptor. Hence, two interacting partners complementary labelled with the respective dyes, induce FRET once they are tightly bound to each other and the red shifted acceptor emission is observed even if the donor is exclusively excited. With the time resolved FRET-technology now available for the TriStar2S the sensitivity of FRET-studies is dramatically enhanced. Time resolved FRET can be applied for FRET assays featuring an acceptor dye with long lived fluorescence decays, such as e.g. the CisBio HTRF®-kit. After excitation of the donor with a short light-pulse, the acceptor emission is only recorded after a tuneable time-delay of 50-300μs. This way, exclusively the acceptor emission is recorded and potential background emission, which typically decays with time constants in the nanosecond time regime, is effectively suppressed.

Thanks to the integration of the TR-FRET modality, the TriStar2S becomes an even more versatile tool for every laboratory which expects best sensitivity, highest flexibility and outstanding quality at an affordable price. The TriStar2S offers the following measurement options:

  • Luminescence
  • UV-Absorbance
  • UV-Fluorescence
  • Time Resolved Fluorescence (TRF)
  • BRET-Luminescence
  • Absorbance spectral scanning (UV-VIS)
  • FRET
  • Time Resolved FRET (TR-FRET/HTRF)
  • VIS-Absorbance
  • Fluorescence
  • Fluorescence excitation scanning

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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.MonochromatorHTRFDyesFRET

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