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TriLink BioTechnologies expands small RNA sequencing portfolio with new primers

TriLink BioTechnologies

Two New sets of barcode convert primers are optimized for Ion Torrent sequencers

14 Oct 2019
Edward Carter
Publishing / Media

Industry news

TriLink BioTechnologies introduced two new primer sets for its CleanTag® kit. The new Ion Torrent Convert barcode primer sets enable small RNA library preparation on the sequencer platform from Thermo Fisher Scientific. TriLink’s CleanTag Small RNA Library Prep Kit has been in use for several years with Illumina sequencers.

CleanTag streamlines and improves next-generation library preparation in a number of ways. Its proprietary formulation includes modified adapters that block adapter-dimer formation – one of the biggest challenges in micro RNA (miRNA) library prep. Adapter-dimers, which are formed with most kits, reduce usable sequencing reads and must be removed. This has typically required a cumbersome gel purification step, which adds time to the process and reduces the amount of RNA available for research. By enabling automated, bead-based separation, CleanTag delivers a simplified, cleaner workflow, retaining high sensitivity and the ability to work with low-abundance RNA input as well as at the single-cell level. Additionally, utilizing the index primers, more than one sample can be sequenced at a time for significant cost savings.

CleanTag is more sensitive and easier to use than other options on the market and is also faster, enabling miRNA preparation in a day, with fewer steps than other approaches. Even with samples containing low-abundance miRNA, such as blood or plasma, CleanTag delivers superior results.

Nicolas Crapoulet, Ph.D., specialist in Molecular Genetics at the Vitality Health Network’s University Hospital Center in New Brunswick, Canada, comments, “Prior to adopting CleanTag, we had quality and consistency issues with available library prep kits. CleanTag’s low adapter-dimer feature has allowed us to eliminate the purification step that reduces the amount of target. Now we can sequence every sample we get. There is less wasted sample with CleanTag and we get more data from each sample.”

Primers provided in each index set for Ion Torrent includes the RT primer, 12 indexed forward-PCR primers and a reverse-PCR primer. A total of 24 unique indexes are available in the two new sets.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.SequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.

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TriLink BioTechnologies expands small RNA sequencing portfolio with new primers