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Translate Neuroscience Discovers into Therapeutics: Join Sigma-Aldrich at Neuroscience 2014

Sigma-Aldrich Supelco
3 Nov 2014
Sarah Thomas
Associate Editor

Industry news

Join Sigma-Aldrich at the Society for Neuroscience 44th Annual Meeting (Booth 1223) to discover solutions for drug discovery research, from target identification and validation through clinical trials. The company’s comprehensive offering will strengthen and support your neuroscience research by ensuring greater accuracy, reproducibility and confidence.

Highlighted Solutions:
Sigma CRISPR – RNA-guided genome editing
CompoZr® Zinc Finger Nuclease Technology – Genome editing
Small Molecule Drug-like Compounds – Modulate protein targets
Prestige Antibodies – Most characterized antibodies commercially available
GE Life Sciences Consumables – Now available from Sigma-Aldrich

Exclusive In-Booth Events:

CRISPR Corner
Sunday, November 16 11:00 AM
Monday, November 17 11:00 AM
Tuesday, November 18 11:00 AM

The CRISPR Craze continues to take the research world by storm. This two-part session will include a brief introduction on the mechanism of how type II CRISPR/Cas9 site-specific nuclease target specific DNA sequences and how it is applied for genome editing. The current Sigma products, including WT Cas9, paired Cas9 nickases, mRNA for transgenic work, CRISPR controls and lentiviral CRISPRs are described.

The next session will describe techniques to create and validate genetically modified cell lines. Also, a case study is presented on how the paired Cas9 nickases can increase specificity and reduce off-targets. Similar to Sigma’s CompoZr ZFNs, the paired nickase approach increases the target site length by using paired gRNAs. Finally, more details on lentiviral CRISPRs for knocking out genes to screen compounds and pathways is provided.

Principles and Advances in Quantitative Western Blotting
Over the past decade, improved detection methods and software have brought quantitative analysis to Western Blotting. Sigma-Aldrich and GE Life Sciences partner for this presentation to guide and inspire beginners and experts toward successful Western Blotting results. We will take you through the Western blotting workflow and show examples of typical applications as well as new approaches to help you improve your methods and obtain better quantitative data. Monday, November 17 at 3:00 PM

Plus, test your neuroscience knowledge with Sigma’s Neuroscience Trivia Bowl - the winner of each round will win a Gift Bag full of goodies!

Links

Sigma-Aldrich SupelcoCompany website

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NeuroscienceNeuroscience research investigates the neurological mechanisms underlying behavior, neurodegenerative diseases, and other brain conditions. Learn about the innovative technologies for bioimaging, electrophysiology, cell culture, chromatography and other techniques used in this field.Genome EditingGenome editing involves altering the DNA of an organism, which holds promise for disease treatment, agriculture, and gene therapy. Techniques like CRISPR-Cas9 are revolutionizing this field, enabling precise genetic modifications. Explore genome editing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Blotting

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Translate Neuroscience Discovers into Therapeutics: Join Sigma-Aldrich at Neuroscience 2014