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哺乳动物细胞中同时提取蛋白和Total RNA成为可能！TaKaRa Protein & RNA Extraction Kit for Mammalian Cultured Cells
13 Jul 2007Meng Ye
Student / Graduate
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本制品是在同一样品中同时高效快速提取Total RNA和Total Protein的试剂盒（简称PAREx法)。本试剂盒的操作过程不用使用苯酚、氯仿，使用本试剂盒提取的Total RNA和Total Protein可用于siRNA的效果分析、RNA/蛋白质的表达分析等。
通常分析经过各种药物处理（或经siRNA Transfection）后的动物培养细胞中的靶mRNA或蛋白质的表达差异时，需要将培养细胞分成两份，或者进行两次培养，一份用于提取Total RNA，另一份用于提取Total Protein，这样实验操作复杂烦琐，实验数据误差较大。本试剂盒可以从同一样品中同时提取Total RNA和蛋白质，方便了实验操作，提高了实验可信度。
本试剂盒使用含非离子性表面活性剂的Cell Lysis Buffer裂解细胞，然后使用盐析法将染色体DNA和细胞残渣除去。
实验操作只需15分钟，便可以从1×103 ~ 1×107个培养细胞中制备含Total RNA和Total Protein的可溶性溶液，
此溶液可直接用于蛋白质电泳和Western Blotting分析等。此溶液经Isopropanol*1沉淀回收Total RNA，再经RNA Preparation Water溶解后的Total RNA溶液*2可用于Real Time RT-PCR法进行mRNA的表达分析等。
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.