Top Six Innovations in HPLC Columns

31 Oct 2014
Lois Manton-O'Byrne, PhD
Executive Editor

Editorial article

1.Raptor™ Biphenyl LC Column - Restek

The Raptor columns comprise of superficially porous particles (also known as SPP or “core-shell” particles) that enable peak separation and faster analysis times without expensive UHPLC instrumentation.


2. Chromolith® High Resolution HPLC Columns - Merck Millpore

Chromolith® columns possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. The high permeability and porosity of the silica skeleton and the resulting low back pressure allow for more flexible flow rates compared to particulate columns.


3. Kinetex® EVO Core-Shell Columns - Phenomenex

The EVO C18 columns are an extension of the PhenomenexKinetex core-shell line, which delivers improved results, increased productivity, easy transferability and significant cost savings compared to traditional HPLC/UHPLC media.


4. XBridge® HPLC Columns - Waters

XBridge® HPLC Columns include a selection of 10 general-purpose and application-specific sorbents that cover a wide range of particles sizes for analytical and preparative HPLC applications.


5. ZORBAX StableBond - Agilent Technologies

The ZORBAX 1.8 μm HPLC columns feature improved packing processes to achieve stability up to 1200 bar for use with the Agilent 1290 Infinity LC and other UHPLC instruments. They are available in more than 12 phases, plus HILIC.


6. Acclaim® 120 C18 Reversed-Phase LC Column - Thermo Fisher Scientific

The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.
Top Six Innovations in HPLC Columns