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ThinCert cell culture inserts as a useful tool for COVID-19 research

Greiner Bio-One GmbH
23 Jul 2020
Georgina Wynne Hughes
Editorial Assistant

Industry news

Greiner Bio-One provide insight into the recently published paper ‘SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells’ and a summarize the most important results and their implications.

Context

Coronavirus infections caused by SARS-CoV (2002/2003), MERS-CoV (since 2012) and SARS-CoV-2 (currently) have severely affected global health in the 21st century. Although being closely related, these three viruses differ in their contagiousness – SARS-CoV-2 shows by far the highest human-to-human transmission. Consequently, global research focuses on mechanisms underlaying the human SARS-Cov-2 infection and pathways the virus uses to enter the human body.

Summary of study results

In this study, the authors utilize ThinCert™ permeable supports to cultivate bronchial cells at the air liquid interface and subsequently characterize their transcriptome. From their experimental results the authors could conclude that mainly a transient secretory cell type seems to be targeted by the SARS-CoV-2 virus. Furthermore, the authors could presume that the specific gene expression patterns of these cells including the expression of TMPRSS2, ACE2 and genes of RHO GTPase pathways are important factors for their increased vulnerability to SARS-CoV-2.

Conclusion and relevance

This study provides a rich resource for future research on the COVID-19 pathogenesis and emphasizes the advantage of utilizing in vitro tissue models in addition to real lung tissue. Permeable membrane supports such as ThinCert™ cell culture inserts are the first-choice if organotypic, tissue-like cell culture conditions are required. The opportunity to cultivate cells at the air-liquid-interface qualifies these devices for the generation of fully differentiated airway-epithelia in vitro and their application in COVID-19 research.

The full publication can be accessed here.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.CoronavirusCoronaviruses are a large family of viruses that can cause illnesses ranging from the common cold to more severe diseases like COVID-19. Research into the biology, transmission, and treatment of coronaviruses has become a global priority, particularly since the emergence of SARS-CoV-2. Understanding the virus�s structure and behavior is essential for developing vaccines, diagnostics, and antiviral treatments. Explore our peer-reviewed product directory to discover the latest research tools, diagnostic tests, vaccines, and treatments for coronavirus, compare products, read reviews, and receive pricing directly from manufacturers.Covid-19COVID-19, caused by the SARS-CoV-2 virus, has led to a global health crisis with profound impacts on societies and economies. Research into diagnostics, vaccines, treatments, and understanding viral mechanisms continues to evolve. The pandemic has accelerated innovations in healthcare and biopharmaceuticals, including novel vaccine platforms and rapid testing technologies. Browse our peer-reviewed product directory to find top COVID-19 testing kits, research tools, vaccines, and treatments, compare products, read reviews, and receive direct pricing from manufacturers.

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ThinCert cell culture inserts as a useful tool for COVID-19 research