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Thermo Fisher Scientific Unveils Gibco ExpiSf System

Thermo Fisher Scientific

The novel insect protein expression system promises superior protein yields, consistent results, a streamlined workflow and scales from discovery to production

3 May 2018
Han Yin
Administrator / Office Personnel

Product news

Historically, undefined insect media components such as yeastolate have made it challenging to achieve consistent results and protein yields across multiple experiments. These factors can significantly slow vaccine development at a time when manufacturers are under pressure to develop vaccines faster and more efficiently. Focused on removing barriers to vaccine development and production, Thermo Fisher Scientific has announced its Gibco ExpiSf system, the first-ever chemically defined insect protein expression system.

The revolutionary ExpiSf system brings together the company’s Gibco media technology, Lipofectamine transfection technology and molecular biology expertise to deliver three times more protein and consistent results. Unlike other systems, ExpiSf has been optimized for the generation of high-quality baculovirus using a suspension-based transfection protocol that delivers protein in half the time compared to traditional insect platforms. The superior performance enables customers to realize significant time savings without sacrificing quality and reproducibility across experiments.

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“The new ExpiSf Expression System offers a convenient virus generation and protein expression cell line in only two steps, for both secreted and intracellular proteins,” commented Xuan Droz, Ph.D., project leader, Redbiotec AG. “We predict that using the ExpiSf system will save us about 30 percent in the time taken for protein expression and make cell maintenance simpler.”

Available now, ExpiSf has many advantages over alternative insect expression platforms:

  • > 300 percent higher protein yield: In performance tests against five competing insect media, the ExpiSf System demonstrated greater protein expression – delivering 3x more protein.
  • Consistent results: Chemically defined, animal-origin free, yeastolate-free growth medium supports consistent cell growth and protein expression, run after run.
  • One optimized system: Fully-integrated platform removes the need to optimize individual components, saving time and cost.
  • Streamlined workflow: The ExpiSf system simplifies baculovirus production to deliver protein in half the time, 6-10 days, compared to 10-20 days via classical techniques.
  • Scales from discovery to production: The scalability of ExpiSf system accelerates development by delivering equivalent cell growth and protein volumetric yield from small to large scale, making the system well-suited for both discovering new therapies and producing them in large quantities.

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“The ExpiSf Expression System is the newest member of the Expi family delivering superior protein expression and consistent performance in Sf9 cells,” commented Amy Butler, vice president and general manager, Cell Biology at Thermo Fisher Scientific. “ExpiSf is the first-ever chemically defined insect expression system that enables our customers to streamline vaccine development from the bench to the clinic. The system is also suitable for applications in basic research where protein structure and/or function is being studied, for example, in structural biology.”

ExpiSf System Components:

  • ExpiSf Expression System Starter Kit - Includes all of the reagents necessary to generate recombinant baculovirus and express proteins with the system.
  • ExpiSf CD Medium - A GMP-manufactured, chemically defined, yeastolate-free, animal origin-free (AOF) high-density insect growth medium.
  • ExpiSf9 Cells - ExpiSf9 is a robust cell line derived from Sf9 cells and adapted for high-density suspension culture in ExpiSf CD Medium.
  • ExpiSf Enhancer – A chemically defined protein expression enhancer that is specifically matched to ExpiSf CD Medium to deliver significantly higher protein yields.
  • ExpiFectamine Sf Transfection Reagent – a next-generation cationic lipid-based reagent for efficient transfection of DNA from insect cells using a faster, simpler protocol than alternative options.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. 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Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.VirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Lab EssentialsLab essentials include laboratory consumables that are vital to the operation of all laboratories.Vaccine DevelopmentVaccine development focuses on creating immunizations to protect against infectious diseases. The process involves identifying antigens, developing and testing vaccine efficacy, and ensuring safety through preclinical testing and multiple clinical trial phases. Scientists use various approaches, including weakened or inactivated viruses, protein subunits, or mRNA. Advanced platforms and rapid diagnostic tools aid in vaccine research and testing. Explore vaccine development solutions in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers. ManufacturingManufacturing is the production of merchandise for use or sale using labor and machines, tools, chemical and biological processing, or formulation.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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