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Thermo Fisher Scientific releases new and improved automation-friendly kit for ribosomal RNA depletion from bacteria

Thermo Fisher Scientific
28 May 2020
Georgina Wynne Hughes
Editorial Assistant

Product news

The Invitrogen™ RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit, offered by Thermo Fisher Scientific, is a new and improved automation-ready kit for depletion of ribosomal RNA from bacteria. Using the kit, researchers can improve RNA-sequencing results with efficient bacterial transcriptome enrichment with probe-based depletion of ribosomal RNA from bacterial total RNA enabling sequencing more RNA.

Clark Bloomer, Project Manager at Genomics Core, researching microbiome said: “We were very encouraged to confirm that 95 percent of NGS reads were non-rRNA transcripts when trailing the RiboMinus Bacteria 2.0 Transcriptome Isolation Kit for rRNA depletion of E. coli when trialing the new product.”

Invitrogen RiboMinus™ products use a novel magnetic bead–based purification technology to enrich the RNA transcript spectrum by selective depletion of rRNA transcripts from total RNA. The RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit now includes 5S targets and over 100 probe designs designed to yield improved rRNA depletion and achieve consistent data.

“We are committed to always improving and adding value to our kits,” said Jeff Franz, director of Product Management of the sample preparation business at Thermo Fisher Scientific. “The latest addition to the portfolio of Invitrogen™ RiboMinus™ isolation kits offer researchers and labs a solution that is designed to increase more targets, more consistency, and better chemistry to enrich the RNA transcript spectrum. The bacteria kit is designed to also work on KingFisher instruments which enable labs to spend less time prepping their samples and more time focusing on downstream analysis.”

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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.BacteriaBacteria are microorganisms that play critical roles in the environment, human health, and biotechnology. Understanding bacterial genetics, growth, and metabolism is essential for developing antibiotics, vaccines, and improving industrial processes. Browse our peer-reviewed product directory to find the best bacterial research tools, compare products, check reviews, and get pricing directly from manufacturers.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.

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Thermo Fisher Scientific releases new and improved automation-friendly kit for ribosomal RNA depletion from bacteria