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Thermo Fisher Scientific Offers New Reagents to Study Kinases

27 Jan 2011
Sarah Sarah
Marketing / Sales

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Thermo Fisher Scientific introduces the Thermo Scientific Pierce Kinase Enrichment Kits. These kits use novel ATP- and ADP-based probes developed by AcitvX Biosciences to covalently modify active sites of ATPases, including kinases, chaperones and metabolic enzymes.

The probes feature a desthiobiotin tag, a biotin analog, attached to the nucleotide. The nucleotide binds to the active site of the kinase, whereas the desthiobiotin tag allows for capture and enrichment of the bound kinase. The desthiobiotin tag binds less tightly to biotin-binding proteins, making the capture bond easily reversible by biotin displacement, low pH or heat. The isolated proteins then can be further studied by Western blot analysis or mass spectrometry. The system is useful for inhibitor profiling and IC50 determinations.

The Pierce® Kinase Enrichment Kit is available with either an ActivX ATP- or ADP-based probe and includes all labeling and enrichment reagents. Both ActivX Probes are also available separately.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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Thermo Fisher Scientific Offers New Reagents to Study Kinases