Thermo Fisher Scientific Launches Comprehensive Line of RNA Purification Kits to Isolate Biomolecules from Diverse Samples

8 Oct 2007

Product news

Thermo Fisher Scientific Inc., the world leader in serving science, has announced the launch of its SurePrep™ RNA Purification Kits, which enable the capture of all sizes of RNA molecules from biological samples. This technology provides purified full-length large RNAs as well as small RNAs such as microRNA (miRNA) and short-interfering RNA (siRNA) that are being investigated as key components in regulating gene expression in signalling pathways, cell death, organ development, metabolism, and disease states.

SurePrep Kits are versatile, isolating RNA from a wide variety of tissues and cell types, and they save time in the lab by reducing the number of steps involved in RNA purification. The SurePrep line utilizes spin-column chromatography with a proprietary resin for capturing RNA molecules from plant and animal tissues, cultured cells, fungi, blood, bacteria and yeast. The offering includes the TrueTotal™ and Small RNA purification kits, which serve a wide range of applications.

The TrueTotal Kit is universal. It enables the extraction of all sizes of RNA, including large messenger RNA, ribosomal RNA as well as small RNA species (less than 200 nucleotides) such as transfer RNA and miRNA. The total volume of RNA eluted from the TrueTotal RNA purification kit is sufficient to perform applications involving both large and small RNA. The SurePrep Small RNA Purification Kit provides a rapid and efficient method to enrich small RNA (less than 200 nucleotides).

Other SurePrep kits will be introduces soon as specific procedures such as isolating nuclear and cytoplasmic RNA, purifying RNA from leucocytes and urine-bacteria RNA, and recovery of RNA, DNA and protein from the same biological sample.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
Thermo Fisher Scientific Launches Comprehensive Line of RNA Purification Kits to Isolate Biomolecules from Diverse Samples