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Thermo Fisher Scientific Introduces Next-Generation Immunoaffinity Sample Preparation Solution for Downstream Mass Spectrometry

1 Oct 2012

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Thermo Fisher Scientific Inc., the world leader in serving science, has introduced its Thermo Scientific Mass Spectrometric Immunoassay (MSIA) pipette tips, a collection of immunoaffinity sample preparation products.

Robust MSIA™ technology delivers a simplified way to enrich target proteins for downstream mass spectrometric analysis. “Our new MSIA pipette tips provide scientists with an easier immunoaffinity approach that yields better and more reproducible quantification results,” said Clive Wingar, business director, affinity & automation consumables, Thermo Fisher Scientific. “From sample preparation and quantification to data analysis, top-down mass spectrometry-based quantitative proteomic workflow is simplified with the automated MSIA immunoaffinity technology, and it is further enhanced when using the highly sensitive Thermo Scientific TSQ Vantage Triple Stage Quadrupole Mass Spectrometer.”

MSIA tips feature a 300 µl pipette tip embedded with a proprietary, highly porous immunoaffinity column that provides increased sensitivity with improved reproducibility and sample throughput. Benefits include:

• Enhanced antibody/antigen-binding kinetics that allow enrichment down to very low levels (femtomoles) for downstream mass spectrometric protein/peptide quantification;
• Improved signal-to-noise ratios due to increased specificity and target recovery as compared to conventional bead-based methods;
• Fast and flexible sample throughput with manual multichannel or fully automated 96-well processing in less than 30 minutes;
• Customizable assay that meets different biomarker research needs.

The new MSIA D.A.R.T.’S® (Disposable Automation Research Tips) are compatible with the Thermo Scientific Versette Automated Liquid Handler providing immuno-enrichment processing in 96-well format. In addition, the new Thermo Scientific Finnpipette Novus i 8 and 12-channel Electronic Pipettes (for immuno-precipitation) and Adjustable Pipette Stand are specifically designed to work with MSIA protocols where high sample throughput is not required.

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Thermo Scientific Versette

Thermo Fisher Scientific

The Thermo Scientific™ Versette is a compact automated liquid handler that offers 96- or 384-channel pipetting heads and user friendly programming options. Its reliable performance and compact size make it a flexible and convenient liquid handling solution for many kinds of laboratories. Versette is designed to perform a variety of liquid handling tasks for a wide range of applications including high-throughput immunoaffinity sample preparation with Mass Spectrometric Immunoassay (MSIA*) pipette tips. It also supports 96/384 plate replication, plate stamping and serial dilution procedures.Key Features: 96-and 384-channel pipetting heads Handle a total volume range of 0.5-300 µl All pipetting heads include RFID tags for self-identify, and utilize Thermo Scientific D.A.R.T.s tips Compact Size The six position stage uses a unique dual-level design to minimise space requirements Fits on standard benches and biological hoods User-friendly programming options: Simple pipetting procedures can be created using visual on-board graphical display For more complex protocols and advanced editing Thermo Scientific ControlMate software is available

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunoprecipitationMSIABinding KineticsImmunoaffinity

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